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Ontogeny and localization of the a-, β-, and δ-crystallin antigens during lens development in mallard embryos
| Content Provider | Scilit |
|---|---|
| Author | Brahma, Samir K. Starre, Henk Van Der |
| Copyright Year | 1982 |
| Description | Journal: Current Eye Research The ontogeny of α-, β-, and δ-crystallin antigens in the developing lens of mallard (Anaa platyrhynchos) was investigated by indirect immunofluorescence using specific antibodies to mallard α-, β-, and δ-crystallins raised in rabbits. The IgG fraction from each antiserum was isolated by affinity chromatography usign Protein A Sepharose CL-4.B. Fluoresceine (FITC) or rhodamine (TRITC) conjugated goat anti rabbit (GAR) γ-globulin was used as the secondary antibody. The results show that α-, β-, and δ-crystallins appear simultaneously in the developing mallard lens and are detectable from 66 hr (stage 15/16). This situation is different from the chick where δ-is known to appear first followed by β-, and α-. This could be due to species variation. In the epithelium, however, as in the chick, δ-emerges first followed by β-, and α-but rather late when compared with the chick and this seems probably due to a difference in the incubation time between the two species. Results from immunofluorescence and Tris-SDS gel electrophoresis demonstrate that αA subunit of the α-crystallin appears much earlier than the αB subunit. We also found that the newly discovered ε-crystallin (18) appears long after the three major crystallin classes. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/02713688209019995 |
| Ending Page | 672 |
| Page Count | 10 |
| Starting Page | 663 |
| ISSN | 02713683 |
| e-ISSN | 14602202 |
| DOI | 10.3109/02713688209019995 |
| Journal | Current Eye Research |
| Issue Number | 10 |
| Volume Number | 2 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1982-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Current Eye Research Microscopic Research Mallard Embryos Crystallin Antigens Lens Development |
| Content Type | Text |
| Subject | Ophthalmology Sensory Systems Cellular and Molecular Neuroscience |
