Rapid assessment of early glycation products by mass spectrometry

Content Provider	Scilit
Author	Prabhakaram, Malladi Smith, J. B. Ortwerth, B. J.
Copyright Year	1996
Description	Journal: IUBMB life In order to detect the early glycation products, we have reacted a model peptide (t‐boc‐lys‐ala‐ala) with L‐threose (a degradation product of ascorbic acid) and analyzed the reaction products by a combination of HPLC and mass spectrometry. Amino group modification, as observed by a fluorescamine assay, indicated complete modification after 3 days of incubation with a 10‐fold excess of threose. As much as 60% of the adducts were acid labile and only 4% of the adducts could be observed by amino acid analysis. However, Fast atom bombardment mass spectrometry (FABMS) of the samples incubated for 6 hr showed relative molecular masses consistent with the formation of adducts corresponding to the addition of one and two molecules of L‐threose to the peptide. Likewise, samples incubated for 12 hr showed peptide adducts with two and three L‐threoses. The number of threose molecules added to the peptide was also confirmed from the FABMS analysis by using [1‐13C]‐threose as the glycating agent.
Related Links	https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1080/15216549600201802
Ending Page	325
Page Count	11
Starting Page	315
e-ISSN	15216551
DOI	10.1080/15216549600201802
Journal	IUBMB life
Issue Number	2
Volume Number	40
Language	English
Publisher	Informa UK Limited
Publisher Date	1996-10-01
Access Restriction	Open
Subject Keyword	Journal: IUBMB life Analytical Chemistry Mass Spectrometry Maillard Reaction Non‐enzymatic Glycation Advanced Glycation End Products (ages)
Content Type	Text