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14-3-3 Family Members Act Coordinately to Regulate Mitotic Progression
| Content Provider | Scilit |
|---|---|
| Author | Dalal, Sorab N. Yaffe, Michael B. DeCaprio, James A. |
| Copyright Year | 2004 |
| Description | Journal: Cell Cycle The mitosis promoting phosphatase, cdc25C, is a target of both the DNA replication and DNA damage checkpoint pathways. These pathways regulate cdc25C function, in part, by promoting the association of cdc25C with 14-3-3 proteins, which results in the retention of cdc25C in the cytoplasm. To determine which 14-3-3 proteins were required to regulate cdc25C function, we tested the ability of various 14-3-3 family members to form a complex with and negatively regulate cdc25C in human cells. Two 14-3-3 family members, 14-3-3? and 14-3-3?, specifically formed a complex with cdc25C but not with the 14-3-3 binding defective cdc25C mutant, S216A. In addition, 14-3-3? and 14Å|3Å|3£^ inhibited the ability of cdc25C, but not the S216A mutant, to induce premature chromatin condensation (PCC) in U-2OS cells. These results suggested that the reduction in PCC by 14-3-3? and 14-3-3? was due to inhibition of cdc25C function. In contrast, 14-3-3? was unable to form a complex with cdc25C, but was able to inhibit the ability of both wild type cdc25C and S216A to induce PCC. This suggests that 14-3-3? regulates entry into mitosis independently of cdc25C and 14-3-3? and 14-3-3?. Thus, specific members of the 14-3-3 family of proteins may act coordinately to maintain the DNA replication checkpoint by regulating the activity of different cell cycle proteins. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/cc.3.5.856?needAccess=true |
| Ending Page | 675 |
| Page Count | 6 |
| Starting Page | 670 |
| ISSN | 15384101 |
| e-ISSN | 15514005 |
| DOI | 10.4161/cc.3.5.856 |
| Journal | Cell Cycle |
| Issue Number | 5 |
| Volume Number | 3 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2004-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cell Cycle Cell Biology Family Members Regulate Cdc25c Cdc25c Function Dna Replication |
| Content Type | Text |
| Subject | Cell Biology Developmental Biology Medicine Molecular Biology |
