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Fluorescence Detection of Free Radicals by Nitroxide Scavenging
| Content Provider | Scilit |
|---|---|
| Author | Gerlock, J. L. Zacmanidis, P. J. Bauer, D. R. Simpson, D. J. Blough, N. V. Salmeen, I. T. |
| Copyright Year | 1990 |
| Description | Journal: Free radical research communications The fluorescence quantum yield of 4-(1-napthoyloxy)-2,2,6,6-tetramethylpiperidine-l-oxyl (I) in acetonitrile and hexane is 55 and 30-fold lower, respectively, than those of diamagnetic analogs. Experiments described herein demonstrate that this property makes possible the fluorescence detection of radical scavenging reactions in which the paramagnetic nitroxide-substituted naphthalene is converted to a diamagnetic N-alkoxy derivative. 2-Cyanopropyl free radicals were generated by the thermal decomposition of azobisisobutyronitrile (AIBN) in cyclohexane or in acetonitrile containing 1. The fluorescence intensity of the sample increased proportionally to the decrease in its ESR signal intensity, indicating the conversion of the paramagnetic nitroxide to the diamagnetic product. The linear relationship between the increase in fluorescence intensity and decrease in ESR signal intensity shows that the changes in the fluorescence intensity can serve as a sensitive means for optically detecting radicals. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/10715769009145941 |
| Ending Page | 121 |
| Page Count | 3 |
| Starting Page | 119 |
| ISSN | 87550199 |
| DOI | 10.3109/10715769009145941 |
| Journal | Free radical research communications |
| Issue Number | 1-2 |
| Volume Number | 10 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1990-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Free radical research communications Atomic, Molecular and Chemical Physics Fluorescence Free Radical |
| Content Type | Text |
| Subject | Biochemistry |
