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LncRNA TBX5-AS1 Regulates the Tumor Progression Through the PI3K/AKT Pathway in Non-Small Cell Lung Cancer
| Content Provider | Scilit |
|---|---|
| Author | Qu, Qing-Hai Jiang, Shui-Zheng Li, Xin-Ying |
| Copyright Year | 2020 |
| Description | Journal: OncoTargets and therapy |
| Abstract | Purpose: Long non-coding RNAs (lncRNAs) have been reported to play important roles in tumor biology. In this study, we aimed to investigate the effects of T-box transcription factor 5 antisense RNA 1 (TBX5-AS1) on aggressive phenotypes of non-small cell lung cancer (NSCLC) cells and explore its regulatory pathway. Methods: The expression of TBX5-AS1 in tissues, plasma, and cells was determined by qRT-PCR. Cell viability, proliferation, migration, invasion, and apoptosis were assessed using MTT, colony formation, wound-healing, Transwell, and flow cytometry assay, respectively. Western blot analysis was performed to measure the expression of apoptosis-related proteins. Besides, transfected cells were exposed to PI3K activator (740Y-P) to verify the regulatory pathway. Results: TBX5-AS1 expression was down-regulated in NSCLC tissues, plasma, and cells, and associated with lymph node metastasis and histological grade. Overexpression of TBX5-AS1 inhibited cell viability, colony formation, migration, and invasion, while it promoted apoptosis. Conversely, knockdown of TBX5-AS1 showed the completely opposite results. Additionally, western blot showed that the phosphorylation of PI3K and AKT was stimulated by TBX5-AS1 knockdown and suppressed by TBX5-AS1 overexpression. The addition of 740Y-P in transfected cells reversed the TBX5-AS1-induced inhibition of PI3K and AKT phosphorylation and effects on aggressive phenotypes of NSCLC cells. Conclusion: The study confirmed the down-regulation of TBX5-AS1 in patients with NSCLC and its association with the progression. We innovatively proposed a possible model of TBX5-AS1-mediated gene regulation in NSCLC progression that TBX5-AS1 inhibited the aggressive phenotypes of NSCLC cells through inactivating the PI3K/AKT pathway. This finding provided a novel insight into NSCLC pathogenesis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7431607/pdf https://www.dovepress.com/getfile.php?fileID=60562 |
| Ending Page | 7961 |
| Page Count | 13 |
| Starting Page | 7949 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s255195 |
| Journal | OncoTargets and therapy |
| Volume Number | ume 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: OncoTargets and therapy Non-small Cell Lung Cancer Long Non-coding Rnas T-box Transcription Factor 5 Antisense Rna 1 Aggressive Phenotype Pi3k/akt Pathway |
| Content Type | Text |
| Subject | Pharmacology (medical) Oncology |
