Loading...
Please wait, while we are loading the content...
A comparative study of ribonucleic acid species in yeast-like and mycelial forms ofParacoccidioides brasiliensis
| Content Provider | Scilit |
|---|---|
| Author | Ramírez-Martínez, J. R. |
| Copyright Year | 1971 |
| Description | Journal: Medical mycology A method is described for the isolation of undergraded RNA from yeast-like (Y) and mycelial (M) forms of Paracoccidioides brasiliensis. The method is a combination of various known techniques. Cosedimentation in a sucrose gradient of Y form RNA labelled with$ ^{14}$C-uracil and M form RNA labelled with$ ^{3}$H-uracil showed identical sedimentation behaviour of the 3 major types of RNA. However, one of these types of RNA (light ribosomal RNA) is comparatively more abundant in the M form than in the Y form of P. brasiliensis. In addition to the 3 major sedimenting types of RNA, many other intermediate species of RNA were detected in both Y and M forms by electrophoretic analyses in polyacrylamide gel. A two-band difference was found between electrophoretic patterns of RNA sedimentation samples obtained from Y and M forms. |
| Related Links | https://academic.oup.com/mmy/article-pdf/9/2/157/4584639/9-2-157.pdf |
| Ending Page | 163 |
| Page Count | 7 |
| Starting Page | 157 |
| ISSN | 13693786 |
| DOI | 10.1080/00362177185190351 |
| Journal | Medical mycology |
| Issue Number | 2 |
| Volume Number | 9 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1971-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Medical mycology Paracoccidioides Brasiliensis |
| Content Type | Text |
| Subject | Infectious Diseases Veterinary |
