NDLI: Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

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Adipose tissue-organotypic culture system as a promising model for studying adipose tissue biology and regeneration

Content Provider	Scilit
Author	Toda, Shuji Uchihashi, Kazuyoshi Aoki, Shigehisa Sonoda, Emiko Yamasaki, Fumio Piao, Meihua Ootani, Akifumi Yonemitsu, Nobuhisa Sugihara, Hajime
Copyright Year	2009
Description	Journal: Organogenesis Adipose tissue consists of mature adipocytes, preadipocytes and mesenchymal stem cells (MSCs), but a culture system for analyzing their cell types within the tissue has not been established. We have recently developed “adipose tissue-organotypic culture system” that maintains unilocular structure, proliferative ability and functions of mature adipocytes for a long term, using three-dimensional collagen gel culture of the tissue fragments. In this system, both preadipocytes and MSCs regenerate actively at the peripheral zone of the fragments. Our method will open up a new way for studying both multiple cell types within adipose tissue and the cell-based mechanisms of obesity and metabolic syndrome. Thus, it seems to be a promising model for investigating adipose tissue biology and regeneration. In this article, we introduce adipose tissue-organotypic culture, and propose two theories regarding the mechanism of tissue regeneration that occurs specifically at peripheral zone of tissue fragments in vitro.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/org.5.2.8347?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710525/pdf
Ending Page	56
Page Count	7
Starting Page	50
ISSN	15476278
e-ISSN	15558592
DOI	10.4161/org.5.2.8347
Journal	Organogenesis
Issue Number	2
Volume Number	5
Language	English
Publisher	Informa UK Limited
Publisher Date	2009-04-01
Access Restriction	Open
Subject Keyword	Journal: Organogenesis Biochemical Research Adipose Tissue-organotypic Culture Central Zone Mature Adipocytes Mesenchymal Stem Cells Peripheral Zone Preadipocytes (immature Adipocytes) Three-dimensional Tissue Fragments Tissue Regeneration
Content Type	Text
Subject	Developmental Biology Transplantation Embryology Biomedical Engineering

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