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miR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells
| Content Provider | Scilit |
|---|---|
| Author | Li, Xin Li, Yan Wan, Li Chen, Rui Chen, Fei |
| Copyright Year | 2017 |
| Abstract | MiR-509-5p inhibits cellular proliferation and migration via targeting MDM2 in pancreatic cancer cells Xin Li,1 Yan Li,2 Li Wan,3 Rui Chen,4 Fei Chen5 1Department of Pharmacology, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 2Department of Ophthalmology and Otorhinolaryngology, Lecong Hospital of Guangzhou Medical University, Foshan, 3Department of Laboratory Medicine, The Fourth Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Major Obstetric Diseases in Guangdong Province, Key Laboratory of Reproduction and Genetics in Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, 5Department of Histology and Embryology, School of Basic Sciences, Guangzhou Medical University, Guangzhou, People’s Republic of China Objective: This study aimed to explore the effect of miR-509-5p on pancreatic cancer progression and clarify the underlying mechanisms.Methods: Real-time quantitative reverse transcription polymerase chain reaction was employed to determine miR-509-5p expression in pancreatic cancer tissues and noncancerous adjacent tissues. CCK-8 and Transwell experiments were employed to examine cellular proliferation, migration, and invasion after miR-509-5p mimic or inhibitor transfection. Bioinformatics tools were used to identify the target gene of miR-509-5p, and cotransfection of the target gene and miR-509-5p mimic was performed to determine the effect on the proliferation and migration of pancreatic cancer cells. A xenograft mouse model and histological analysis were also used to test the effect of miR-509-5p on tumor growth in vivo.Results: miR-509-5p expression was dramatically downregulated in pancreatic cancer tissues and in pancreatic cancer cell lines. miR-509-5p mimic markedly inhibited PANC-1 cell proliferation, migration, and invasion. Conversely, miR-509-5p inhibitor promoted PANC-1 cell proliferation, migration, and invasion. Furthermore, the 3'UTR–specific target site luciferase reporter assay also showed that miR-509-5p negatively regulated MDM2 at the post-transcriptional level. miR-509-5p effectively reversed the MDM2 overexpression-induced increase in PANC-1 cell proliferation and invasion. Moreover, miR-509-5p inhibited tumor growth and accelerated cell death in the tumor samples.Conclusions: Our results suggested that miR-509-5p served as a new tumor suppressor via targeting the MDM2 gene, inhibiting pancreatic cancer progression. Keywords: miR-509-5p, pancreatic cancer, MDM2, proliferation, migration, invasion |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5602284/pdf https://www.dovepress.com/getfile.php?fileID=38349 |
| Ending Page | 4464 |
| Page Count | 10 |
| Starting Page | 4455 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s130378 |
| Journal | OncoTargets and therapy |
| Volume Number | 10 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2017-09-01 |
| Access Restriction | Open |
| Subject Keyword | Gastroenterology and Hepatology Invasion Mir-509-5p Migration Pancreatic Cancer Proliferation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
