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MicroRNA-125a-5p regulates cancer cell proliferation and migration through NAIF1 in prostate carcinoma
| Content Provider | Scilit |
|---|---|
| Author | Fu, Yi Cao, Fuhua |
| Copyright Year | 2015 |
| Abstract | MicroRNA-125a-5p regulates cancer cell proliferation and migration through NAIF1 in prostate carcinoma Yi Fu, Fuhua Cao Department of Urology, The Oilfield General Hospital of Daqing, Daqing, People’s Republic of China Background: We investigated the functional roles of microRNA-125a-5p in regulating human prostate carcinoma. Methods: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was conducted to evaluate the gene expression levels of miR-125a-5p in eight prostate cancer cell lines and nine biopsy specimens from patients with prostate cancer. miR-125a-5p was genetically knocked down in prostate cancer cell lines, DU145 and VCaP cells by lentiviral transduction. The effects of miR-125a-5p downregulation on prostate cancer cell proliferation and migration were evaluated by MTT assay and transwell assay, respectively. Direct regulation of miR-125a-5p on its downstream targets, NAIF1, and apoptotic gene caspase-3 were evaluated through dual-luciferase reporter assay, qRT-PCR, and Western blot, respectively. NAIF1 was then ectopically overexpressed in DU145 and VCaP cells to modulate prostate cancer cell proliferation and migration. Finally, the effects of miR-125a-5p downregulation or NAIF1 overexpression on the growth of in vivo prostate cancer xenograft were evaluated. Results: miR-125a-5p was upregulated in prostate cancer cell lines and human prostate carcinomas. Lentivirus induced miR-125a-5p downregulation in DU145 and VCaP cells inhibited prostate cancer cell proliferation or migration. NAIF1 was the direct target of miR-125a-5p, as both gene and protein expression levels of NAIF1, as well as caspase-3 were upregulated by miR-125a-5p. Forced overexpression of NAIF1 had similar antitumor effects as miR-125a-5p downregulation on prostate cancer cell proliferation and migration. In vivo prostate xenograft assay confirmed the tumor-suppressive effect of miR-125a-5p downregulation or NAIF1 overexpression. Conclusion: miR-125a-5p regulates prostate cancer cell proliferation and migration through NAIF1. Keywords: miR-125a-5p, NAIF1, caspase-3, proliferation, migration |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689268/pdf https://www.dovepress.com/getfile.php?fileID=28415 |
| Ending Page | 3835 |
| Page Count | 9 |
| Starting Page | 3827 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s92314 |
| Journal | OncoTargets and therapy |
| Volume Number | 8 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2015-12-01 |
| Access Restriction | Open |
| Subject Keyword | Medical Laboratory Technology Oncology Mir-125a-5p Caspase-3 Proliferation Migration |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
