Pipeline for acquisition of quantitative data on segmentation gene expression from confocal images.

Content Provider	Scilit
Author	Surkova, Svetlana Myasnikova, Ekaterina Janssens, Hilde Kozlov, Konstantin N. Samsonova, Anastasia A. Reinitz, John Samsonova, Maria
Copyright Year	2008
Description	We describe a data pipeline developed to extract the quantitative data on segmentation gene expression from confocal images of gene expression patterns in Drosophila. The pipeline consists of five steps: image segmentation, background removal, temporal characterization of an embryo, data registration and data averaging. This pipeline was successfully applied to obtain quantitative gene expression data at cellular resolution in space and at the 6.5-minute resolution in time, as well as to construct a spatiotemporal atlas of segmentation gene expression. Each data pipeline step can be easily adapted to process a wide range of images of gene expression patterns.
Related Links	http://www.tandfonline.com/doi/pdf/10.4161/fly.6060?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2803333/pdf
Ending Page	66
Page Count	9
Starting Page	58
ISSN	19336934
e-ISSN	19336942
DOI	10.4161/fly.6060
Journal	Fly
Issue Number	2
Volume Number	2
Language	English
Publisher	Informa UK Limited
Publisher Date	2008-03-23
Access Restriction	Open
Subject Keyword	Mathematical and Computational Biology Image Processing Confocal Microscopy Gene Expression Image Segmentation Spatial Registration Background Removal
Content Type	Text
Resource Type	Article
Subject	Insect Science