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Isolation and characterization of a monoclonal antibody containing an extra heavy-light chain Fab arm
| Content Provider | Scilit |
|---|---|
| Author | Boyd, Dan Ebrahimi, Arpa Ronan, Sarah Mickus, Brian Schenauer, Matthew Wang, Jenny Brown, Darren Ambrogelly, Alexandre |
| Copyright Year | 2018 |
| Description | Isolation and characterization of monoclonal antibody (mAb) variants to understand the impact of their structure on function is a typical activity during early-stage candidate selection that contributes to derisking clinical development. In particular, efforts are devoted to characterizing oligomeric variants, owing to their potential immunogenic nature. We report here a mAb variant consisting of a canonical mAb monomer associated in a non-covalent fashion with an antigen-binding fragment (Fab) arm amputated from its Fc domain. The truncated heavy chain is encoded in the cell line genome and is the likely product of a genomic recombination during cell line generation. The addition of the Fab arm results in severe loss of potency, indicating its interaction with the Fab domain of the monomer. The presence of such a variant can easily be mitigated by an adequate purification step. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/19420862.2018.1438795?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5916558/pdf |
| Ending Page | 353 |
| Page Count | 8 |
| Starting Page | 346 |
| ISSN | 19420862 |
| e-ISSN | 19420870 |
| DOI | 10.1080/19420862.2018.1438795 |
| Journal | mAbs |
| Issue Number | 3 |
| Volume Number | 10 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2018-03-20 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Antibody Impurities Cell Line High Molecular Weight Species Monoclonal Antibody Molecular Size Variant Size Exclusion Chromatography Sec-mals |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
