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Determination of target biogenic amines in fish by GC-MS: investigating seafood quality
| Content Provider | Scilit |
|---|---|
| Author | Teixeira, Edgar Noronha, João Paulo Barbosa, Vera Liane Anacleto, Patrícia Maulvault, Ana Luísa Nunes, Maria Leonor Marques, António Diniz, Mario |
| Copyright Year | 2019 |
| Abstract | Introduction: Worldwide there is a strong requirement for food quality and safety [ 1 Alasalvar C , et al. editors. Handbook of seafood quality, safety, and health applications. Blackwell Publishing Ltd.; 2011. ISBN 978-1-4051-8070-2. [Google Scholar] ], with the chemical and biological nature of hazards being the major concern. Fish degradation is a complex phenomenon where a series of events occur simultaneously, influencing each other and beginning immediately at the time of death [ 1 Alasalvar C , et al. editors. Handbook of seafood quality, safety, and health applications. Blackwell Publishing Ltd.; 2011. ISBN 978-1-4051-8070-2. [Google Scholar] ]. Consequently, a reliable fish quality analysis is essential for consumer protection. Thus, the main aim of the present study was to develop an analytical approach based on sample treatment and GC-MS methodologies to detect biogenic amines as products of seafood degradation. Materials and methods: For the GC-MS analysis two Atlantic fish species were selected (Trachurus trachurus and Sarda sarda). The fish were fileted and exposed to room temperature for 9 days (T. trachurus) and 13 days (S. sarda). Samples were collected every day and frozen (-80ºC) for later analysis. Following the periods of exposure, stored samples, were homogenized in 75:25 Methanol: 0,4N HCl. Then, samples were treated and analyzed by GC-MS as described by Richard et al. [ 2 Richard NL , Pivarnik LF , Ellis PC , et al. Impact of Quality Parameters on the Recovery of Putrescine and Cadaverine in Fish Using Methanol-Hydrochloric Acid Solvent Extraction, J AOAC. 2011;94(4):1177–1188. [PubMed], [Web of Science ®] , [Google Scholar] ]. Results: The results show that biogenic amines arise after two days of exposure in analysed fish samples. Following GC-MS analysis, putrescine, tyramine, spermine and spermidine emerged in two days and cadaverine and phenylethylamine after four days of exposure. However, cadaverine, spermine and spermidine decreased below LD (limit of detection) after nine days of exposure showing a different profile over exposure time. Discussion and conclusions: The different biogenic amines profile found is compatible with the bacterial activity in sea food, namely biogenic amines which are usually produced by decarboxylation of free amino acids and transamination of aldehydes and ketones by the action of diverse microorganisms. Moreover, although GC-MS seems to be reliable technique to analyse biogenic amines in seafood samples more analysis should be performed focused on other sample treatment methodologies and cross-checking with other analytical techniques (e.g. LC-MS/MS and HPLC) until validation. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/07853890.2018.1561675?needAccess=true |
| Ending Page | 73 |
| Page Count | 1 |
| Starting Page | 73 |
| ISSN | 07853890 |
| e-ISSN | 13652060 |
| DOI | 10.1080/07853890.2018.1561675 |
| Journal | Annals of Medicine |
| Issue Number | sup1 |
| Volume Number | 51 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-03-29 |
| Access Restriction | Open |
| Subject Keyword | Food Science and Technology Safety Google Scholar Biogenic Sample Treatment Gc Ms Analysis |
| Content Type | Text |
| Resource Type | Synopsis |
| Subject | Medicine |
