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Recent developments in cell-based assays and stem cell technologies for botulinum neurotoxin research and drug discovery

Content Provider	Scilit
Author	Kiris, Erkan Kota, Krishna P. Burnett, James C. Soloveva, Veronica Kane, Christopher Bavari, Sina
Copyright Year	2014
Description	Botulinum neurotoxins (BoNTs) are exceptionally potent inhibitors of neurotransmission, causing muscle paralysis and respiratory failure associated with the disease botulism. Currently, no drugs are available to counter intracellular BoNT poisoning. To develop effective medical treatments, cell-based assays provide a valuable system to identify novel inhibitors in a time- and cost-efficient manner. Consequently, cell-based systems including immortalized cells, primary neurons and stem cell-derived neurons have been established. Stem cell-derived neurons are highly sensitive to BoNT intoxication and represent an ideal model to study the biological effects of BoNTs. Robust immunoassays are used to quantify BoNT activity and play a central role during inhibitor screening. In this review, we examine recent progress in physiologically relevant cell-based assays and high-throughput screening approaches for the identification of both direct and indirect BoNT inhibitors.
Related Links	http://europepmc.org/articles/pmc4478076?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4478076/pdf
Ending Page	168
Page Count	16
Starting Page	153
ISSN	14737159
e-ISSN	17448352
DOI	10.1586/14737159.2014.867808
Journal	Expert Review of Molecular Diagnostics
Issue Number	2
Volume Number	14
Language	English
Publisher	Informa UK Limited
Publisher Date	2014-03-01
Access Restriction	Open
Subject Keyword	Analytical Chemistry Botulinum Neurotoxin Cell-based Assays Embryonic Stem Cells Motor Neurons Drug Discovery High-throughput Screening High-content Imaging Phenotypic Screening
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Molecular Medicine Pathology and Forensic Medicine

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