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Peptides: Purification with Immobilized Enzymes
| Content Provider | Scilit |
|---|---|
| Author | Hamada, Jamel S. |
| Copyright Year | 2005 |
| Description | Affinity chromatography is a powerful separation technique that exploits specific binding properties among biomolecules. For protein purification, the enrichment obtainable in single-step affinity chromatography sometimes exceeds 1000-fold. This high selectivity is due to the vast variety of specific interactions that characterize the functional properties of protein molecules. Like any other affinity chromatography technique, purification of peptides by immobilized enzymes captures the unique interactions between biomolecules (see $Refs.^{[1–3]}$). For example, many enzymes reversibly bind to their organic cofactor molecules and inhibitors. The separation of such enzymes from other proteins can be easily achieved by using a chromatography column containing immobilized inhibitors. Inversely, the enzyme inhibitors can be isolated and purified to a high degree of purity by affinity chromatography using immobilized enzymes. Because affinity chromatography is a most selective technique, it may be used alone as a single chromatography step for peptide purification. However, as many inputmaterial contaminants as possible must be removed prior to using affinity chromatography (e.g., before the affinity chromatography step). For instance, ultrafiltration or GPC is sometimes used concurrently to separate the high-and low-molecular-weight forms while exchanging buffers. In this entry, the focus will be on the use of affinity chromatography to purify peptides when the immobilized ligand is an enzyme. Book Name: Encyclopedia of Chromatography |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2009-1-02373-3&isbn=9780367803766&doi=10.1201/NOE0824727857-261&format=pdf |
| Ending Page | 1228 |
| Page Count | 3 |
| Starting Page | 1226 |
| DOI | 10.1201/noe0824727857-261 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2005-06-06 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Encyclopedia of Chromatography Analytical Chemistry Protein Functional Purification Immobilized Contaminants Step Affinity Chromatography |
| Content Type | Text |
| Resource Type | Chapter |
