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Detection of Double-Stranded RNAs
| Content Provider | Scilit |
|---|---|
| Author | Narayanasamy, P. |
| Copyright Year | 2001 |
| Description | Infected plants may have double-stranded-(ds)-RNAs when a) the infecting viral genome is ds-RNA, as in phytoreoviruses and cryptoviruses, or b) ds-RNA is produced as a replicating form during the process of replication of single-stranded (ss)-RNA viruses. In the case of certain viruses, such as velvet tobacco mottle virus, ds-RNA may accumulate during replication. This technique of detecting the ds-RNA is useful for the early and rapid recognition of virus infection. The presence of ds-RNA can be detected by either polyacrylamide gel electrophoresis (PAGE) or antiserum reaction with ds-RNA. The quantity of ds-RNA obtained may vary with host-virus combinations (Valverde et aI., 1986). The ds-RNA may be isolated and labeled or cloned as cDNA for developing nucleic acid probes. Book Name: Plant Pathogen Detection and Disease Diagnosis |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2006-0-13620-6&isbn=9780429153235&doi=10.1201/9781482270952-17&format=pdf |
| Ending Page | 412 |
| Page Count | 4 |
| Starting Page | 409 |
| DOI | 10.1201/9781482270952-17 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2001-08-28 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Plant Pathogen Detection and Disease Diagnosis Rna Viruses Polyacrylamide Gel Double Stranded Detecting Ds Rna |
| Content Type | Text |
| Resource Type | Chapter |
