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Biological and clinical relevance of quantitative global methylation of repetitive DNA sequences in chronic lymphocytic leukemia
| Content Provider | Scilit |
|---|---|
| Author | Fabris, Sonia Bollati, Valentina Agnelli, Luca Morabito, Fortunato Motta, Valeria Cutrona, Giovanna Matis, Serena Recchia, Anna Grazia Gigliotti, Vincenzo Gentile, Massimo Deliliers, Giorgio Lambertenghi Bertazzi, Pier Alberto Ferrarini, Manlio Neri, Antonino Baccarelli, Andrea |
| Copyright Year | 2011 |
| Description | Global DNA hypomethylation affecting repeat sequences has been reported in different cancer types. Herein, we investigated the methylation levels of repetitive DNA elements in chronic lymphocytic leukemia (CLL), their correlation with the major cytogenetic and molecular features, and clinical relevance in predicting therapy-free survival (TFS). A quantitative bisulfite-PCR Pyrosequencing method was used to evaluate methylation of Alu, long interspersed nuclear elements-1 (LINE-1) and satellite-α (SAT-α) sequences in 77 untreated early-stage (Binet A) CLL patients. Peripheral B-cells from 7 healthy donors were used as controls. Methylation levels (median %5mC) were lower in B-CLLs compared with controls (21.4 vs. 25.9; 66.8 vs. 85.7; 84.0, vs. 88.2 for Alu, LINE-1 and SAT-α, respectively) (p < 0.001). Among CLL patients, a significant association was observed with 17p13.1 deletion (16.8 vs. 22.4; 51.2 vs. 68.5; 52.6 vs. 85.0, for Alu, LINE-1 and SAT-α) but not with other major genetic lesions, IgVH mutation status, CD38 or ZAP-70 expression. Follow-up analyses showed that lower SAT-α methylation levels appeared to be an independent prognostic marker significantly associated with shorter TFS. Our study extended previous limited evidences in methylation of repetitive sequences in CLL suggesting an important biological and clinical relevance in the disease. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.4161/epi.6.2.13528?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3775884/pdf |
| Ending Page | 194 |
| Page Count | 7 |
| Starting Page | 188 |
| ISSN | 15592294 |
| e-ISSN | 15592308 |
| DOI | 10.4161/epi.6.2.13528 |
| Journal | Epigenetics |
| Issue Number | 2 |
| Volume Number | 6 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2011-02-01 |
| Access Restriction | Open |
| Subject Keyword | Medical Laboratory Technology Dna Methyltransferases Line-1 Sat-α Chronic Lymphocytic Leukemia |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Cancer Research |
