The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit

Content Provider	Scilit
Author	Botchkarev, Vladimir V. Rossio, Valentina Yoshida, Satoshi
Copyright Year	2014
Description	Polo-like kinases are important regulators of multiple mitotic events; however, how Polo-like kinases are spatially and temporally regulated to perform their many tasks is not well understood. Here, we examined the subcellular localization of the budding yeast Polo-like kinase Cdc5 using a functional Cdc5-GFP protein expressed from the endogenous locus. In addition to the well-described localization of Cdc5 at the spindle pole bodies (SPBs) and the bud neck, we found that Cdc5-GFP accumulates in the nucleus in early mitosis but is released to the cytoplasm in late mitosis in a manner dependent on the Cdc14 phosphatase. This Cdc5 release from the nucleus is important for mitotic exit because artificial sequestration of Cdc5 in the nucleus by addition of a strong nuclear localization signal (NLS) resulted in mitotic exit defects. We identified a key cytoplasmic target of Cdc5 as Bfa1, an inhibitor of mitotic exit. Our study revealed a novel layer of Cdc5 regulation and suggests the existence of a possible coordination between Cdc5 and Cdc14 activity.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/15384101.2014.953882?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4612440/pdf
Ending Page	3270
Page Count	11
Starting Page	3260
ISSN	15384101
e-ISSN	15514005
DOI	10.4161/15384101.2014.953882
Journal	Cell Cycle
Issue Number	20
Volume Number	13
Language	English
Publisher	Informa UK Limited
Publisher Date	2014-10-15
Access Restriction	Open
Subject Keyword	Cell Biology Fear, Cdc Fourteen Early Anaphase Release Men, Mitotic Exit Network Polo-like Kinase Mitosis Mitotic Exit
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Biology