NDLI: Laboratory Molecular Methodologies to Analyze DNA Methylation

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Laboratory Molecular Methodologies to Analyze DNA Methylation

Content Provider	Scilit
Author	Monk, David Kelsey, Gavin
Copyright Year	2018
Description	DNA methylation is among a growing list of epigenetic modifications that have important roles in regulating gene expression and therefore is involved in a broad range of biological processes and diseases. Epigenetic profiles, including DNA methylation, are tissue-specific, contribute to cell identity, and can be dynamic during development. In this chapter, we discuss genome-wide and locus-specific techniques that divide into three main categories: restriction enzyme digestion, affinity-based analysis, and bisulfite modification. In general, candidate biomarkers are discovered using large-scale, genome-wide sequencing and/or microarray-based profiling strategies, with confirmation performed using various PCR-based methods. We also describe the emerging state-of-the-art technologies for single-cell epigenome profiling and the integration of various protocols for innovative multi-omic analyses that allow researchers to disentangle cell and tissue heterogeneity that is particularly relevant for reproductive epigenomics. Book Name: Epigenetics and Assisted Reproduction
Related Links	https://content.taylorfrancis.com/books/download?dac=C2016-0-31461-4&isbn=9781315208701&doi=10.1201/9781315208701-5&format=pdf
Ending Page	97
Page Count	29
Starting Page	69
DOI	10.1201/9781315208701-5
Language	English
Publisher	Informa UK Limited
Publisher Date	2018-09-03
Access Restriction	Open
Subject Keyword	Book Name: Epigenetics and Assisted Reproduction Reproductive Biology Dna Methylation Gene Expression Epigenetic Tissue Genome Wide Epigenome Profiling
Content Type	Text
Resource Type	Chapter

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