Detection ofMycoplasma synoviaeby polymerase chain reaction

Content Provider	Scilit
Author	Zhao, Shaohua Yamamoto, Richard
Copyright Year	1993
Description	A Mycoplasma synoviae (Ms) species‐specific recombinant subclone, pMS156–20, of approximately 1.1 kbp was partially sequenced. Based on the sequence data, a pair of 25 base primers were synthesized to develop a Ms polymerase chain reaction (Afs‐PCR). The primers amplified target DNA of approximately 1.1 kbp and had excellent specificity over a range of annealing temperature from 60°C to 68°C. The primers amplified 100 pg of 26 strains or isolates of Ms, but not three strains of M. gallisepticum (S6, K810, A5969), 15 other avian Mycoplasma species, and pUC8 plasmid. The minimum amount of target DNA detected by Ms‐PCR was 10 fg, which was 100,000 times more sensitive than the dot blot methodology using Ms recombinant DNA probes. The specificity of Ms‐PCR product detected by gel electrophoresis was confirmed by Southern blot hybridization using an internal probe derived from pMSl56–20.
Related Links	http://www.tandfonline.com/doi/pdf/10.1080/03079459308418941?needAccess=true
Ending Page	542
Page Count	10
Starting Page	533
ISSN	03079457
e-ISSN	14653338
DOI	10.1080/03079459308418941
Journal	Avian Pathology
Issue Number	3
Volume Number	22
Language	English
Publisher	Informa UK Limited
Publisher Date	1993-09-01
Access Restriction	Open
Subject Keyword	Biotechnology and Microbiology Polymerase Chain Reaction Gel Electrophoresis
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Animal Science and Zoology Food Animals