Loading...
Please wait, while we are loading the content...
Similar Documents
Ultrasound Assisted a Peroxisome Proliferator-Activated Receptor (PPAR)γ Agonist-Loaded Nanoparticle-Microbubble Complex to Attenuate Renal Interstitial Fibrosis
| Content Provider | Scilit |
|---|---|
| Author | Wei, Shuping Xu, Chaoli Zhang, Yidan Shi, Zhongqing Wu, Min Yang, Bin |
| Copyright Year | 2020 |
| Description | Objective: To investigate the antifibrotic effect of the combination of a PPARγ agonist-loaded nanoparticle-microbubble complex with ultrasound (US) exposure on renal interstitial fibrosis (RIF). Materials and Methods: Polylactide-co-glycolide (PLGA) nanoparticles were used to load PPARγ agonist (rosiglitazone, RSG) and prepare PLGA-RSG nanoparticles (PLNPs-RSG); then, a novel complex between PLNPs-RSG and SonoVue microbubbles (MBs) (PLNPs-RSG-MBs) was prepared. The size distribution, zeta potentials, RSG-loading capacity and entrapment efficiency were measured, and the release of RSG was assessed using a UV-vis spectrophotometer. The in vitro cytotoxicity and in vivo systemic toxicity assays were performed. The cellular uptake assessment was performed using a confocal laser scanning microscope (CLSM). The in vivo biodistribution assessment was performed using fluorescence imaging with a near-infrared (NIR) imaging system. Furthermore, this complex was administered to a unilateral ureteral obstruction (UUO) rat model with the assistance of US exposure to investigate the antifibrotic effect. Results: This PLNPs-RSG-MBs complex had a size of 2199.5± 988.1 nm and a drug-loading efficiency of 28.5%. In vitro cytotoxicity and in vivo systemic toxicity assays indicated that the PLNPs-RSG-MBs complex displayed excellent biocompatibility. In addition, the complex showed high cellular uptake efficiency in vitro and kidney-targeting ability in vivo. In a UUO rat model, the combination of the PLNPs-RSG-MBs complex with US exposure significantly reduced collagen deposition and successfully attenuated renal fibrosis. Conclusion: The combination of the PLNPs-RSG-MBs complex with US exposure may be a promising approach for the treatment of RIF. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537998/pdf https://www.dovepress.com/getfile.php?fileID=62048 |
| Ending Page | 7327 |
| Page Count | 13 |
| Starting Page | 7315 |
| e-ISSN | 11782013 |
| DOI | 10.2147/ijn.s262052 |
| Journal | International Journal of Nanomedicine |
| Volume Number | 15 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-10-01 |
| Access Restriction | Open |
| Subject Keyword | Ultrasound Nanoparticles Microbubbles Peroxisome Proliferator-activated Receptor Renal Interstitial Fibrosis |
| Content Type | Text |
| Resource Type | Article |
