NDLI: The cadmium–mercaptoacetic acid complex contributes to the genotoxicity of mercaptoacetic acid-coated CdSe-core quantum dots

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The cadmium–mercaptoacetic acid complex contributes to the genotoxicity of mercaptoacetic acid-coated CdSe-core quantum dots

Content Provider	Scilit
Author	Tang, Weikun Fan, Junpeng He, Yide Huang, Bihai Liu, Huihui Pang, Dai-Wen Xie, Zhixiong
Copyright Year	2012
Description	Abstract: Quantum dots (QDs) have many potential clinical and biological applications because of their advantages over traditional fluorescent dyes. However, the genotoxicity potential of QDs still remains unclear. In this paper, a plasmid-based system was designed to explore the genotoxic mechanism of QDs by detecting changes in DNA configuration and biological activities. The direct chemicobiological interactions between DNA and mercaptoacetic acid-coated CdSe-core QDs (MAA–QDs) were investigated. After incubation with different concentrations of MAA–QDs (0.043, 0.13, 0.4, 1.2, and 3.6 µmol/L) in the dark, the DNA conversion of the covalently closed circular (CCC) DNA to the open circular (OC) DNA was significantly enhanced (from 13.9% ± 2.2% to 59.9% ± 12.8%) while the residual transformation activity of plasmid DNA was greatly decreased (from 80.7% ± 12.8% to 13.6% ± 0.8%), which indicated that the damages to the DNA structure and biological activities induced by MAA–QDs were concentration-dependent. The electrospray ionization mass spectrometry data suggested that the observed genotoxicity might be correlated with the cadmium–mercaptoacetic acid complex (Cd–MAA) that is formed in the solution of MAA–QDs. Circular dichroism spectroscopy and transformation assay results indicated that the Cd–MAA complex might interact with DNA through the groove-binding mode and prefer binding to DNA fragments with high adenine and thymine content. Furthermore, the plasmid transformation assay could be used as an effective method to evaluate the genotoxicities of nanoparticles.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368512/pdf https://www.dovepress.com/getfile.php?fileID=12797
Ending Page	2640
Page Count	10
Starting Page	2631
e-ISSN	11782013
DOI	10.2147/IJN.S32029
Journal	International Journal of Nanomedicine
Volume Number	7
Language	English
Publisher	Informa UK Limited
Publisher Date	2012-05-01
Access Restriction	Open
Subject Keyword	Toxicology Maa Cdse Quantum Dots Cadmium-maa Complex Genotoxicity Transformation Assay
Content Type	Text
Resource Type	Article

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