NDLI: Confocal microscopy study of pertussis toxin and toxoids on CHO-cells

Please wait, while we are loading the content...

Confocal microscopy study of pertussis toxin and toxoids on CHO-cells

Content Provider	Scilit
Author	Tan, Yajun Fleck, Roland A. Asokanathan, Catpagavalli Yuen, Chun-Ting Xing, Dorothy Zhang, Shumin Wang, Jun-Zhi
Copyright Year	2013
Description	Pertussis toxin in its detoxified form is a major component of all current acellular pertussis vaccines. Here we report the membrane translocation and internalization activities of pertussis toxin and various pertussis toxoids using Chinese hamster ovary cells and confocal microscopy based on indirect immunofluorescence labeling. Chemically detoxified pertussis toxoids were able to translocate/internalize into cells at the concentration about 1,000 times higher than the native toxin. Pertussis toxoids detoxified with different procedures (glutaraldehyde, glutaraldehyde plus formaldehyde, hydrogen peroxide or genetic mutation) showed differences in fluorescence intensity under the same condition, indicating toxoids from different detoxification methods may have different translocation/internalization activities on cells.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/hv.22795?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3859756/pdf
Ending Page	338
Page Count	7
Starting Page	332
ISSN	21645515
e-ISSN	2164554X
DOI	10.4161/hv.22795
Journal	Human vaccines & immunotherapeutics
Issue Number	2
Volume Number	9
Language	English
Publisher	Informa UK Limited
Publisher Date	2013-02-01
Access Restriction	Open
Subject Keyword	Atmospheric Sciences Cho Cells Confocal Microscopy Detoxification Pertussis Toxin Toxoid Translocation
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology Pharmacology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in