NDLI: Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

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Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

Content Provider	Scilit
Author	Uchida, Takayuki Kaku, Yoshio Hayasaka, Hideyuki Kofuji, Masaya Momose, Naoki Miyazawa, Haruhisa Ueda, Yuichiro Ito, Kiyonori Ookawara, Susumu Morishita, Yoshiyuki
Copyright Year	2019
Description	Background: Endotoxin contamination of dialysate has serious adverse effects on patients undergoing hemodialysis. Therefore, endotoxin activity in dialysate is closely monitored. Limulus amebocyte lysate (LAL) has been used as a reagent to measure endotoxin activity. Here, we investigated the efficacy of an automatic LAL kinetic turbidimetric test (Toxinometer ET-mini) for screening endotoxin activity in dialysate. Methods: In total, endotoxin activity was measured in 110 dialysate samples obtained from several sites within hemodialysis circuits between June 2012 and March 2018. The results were compared with those from a conventional chromogenic substrate LAL test conducted by a clinical examination laboratory. Results: Both the automatic LAL test and the chromogenic substrate LAL test had a minimum detection level of 0.001 endotoxin units (EU)/mL. Endotoxin activity levels measured via the automatic LAL test showed a strongly positive correlation (concordance correlation coefficient: 0.9933; 95% CI: 0.9902–0.9954) and good agreement (mean difference: 0.00±0.01 EU/mL) with those obtained using the chromogenic substrate LAL test. Conclusion: The results suggest that the automatic LAL test may be useful for endotoxin activity screening in hemodialysis facilities.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6789175/pdf https://www.dovepress.com/getfile.php?fileID=53172
Ending Page	433
Page Count	5
Starting Page	429
ISSN	11791470
e-ISSN	11791470
DOI	10.2147/mder.s225246
Journal	Medical Devices: Evidence and Research
Volume Number	12
Language	English
Publisher	Informa UK Limited
Publisher Date	2019-10-01
Access Restriction	Open
Subject Keyword	Integrative and Complementary Medicine Chromogenic Substrate Dialysate Endotoxin Hemodialysis limulus Amebocyte Lysate
Content Type	Text
Resource Type	Article
Subject	Medicine Biomedical Engineering

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Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

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