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Proteomic identification of carbonylated proteins in the kidney of trichloroethene-exposed MRL+/+ mice
| Content Provider | Scilit |
|---|---|
| Author | Fan, Xiuzhen Wang, Gangduo English, Robert D. Khan, M. Firoze |
| Copyright Year | 2013 |
| Description | Trichloroethene (TCE), a common environmental and occupational pollutant, is associated with multiorgan toxicity. Kidney is one of major target organs affected as a result of TCE exposure. Our previous studies have shown that exposure to TCE causes increased protein oxidation (protein carbonylation) in the kidneys of autoimmune-prone MRL+/+ mice, and suggested a potential role of protein oxidation in TCE-mediated nephrotoxicity. To assess the impact of chronic TCE exposure on protein oxidation, particularly to identify the carbonylated proteins in kidneys, female MRL+/+ mice were treated with TCE at the dose of 2 mg/ml via drinking water for 36 weeks and kidney protein extracts were analyzed for protein carbonyls and carbonylated proteins identified using proteomic approaches (2D gel, Western blot, MALDI TOF/TOF MS/MS, etc.). TCE treatment led to significantly increased protein carbonyls in the kidney protein extracts (20 000 g pellet fraction). Interestingly, among 18 identified carbonylated proteins, 10 were found only in the kidneys of TCE-treated mice, whereas other 8 were present in the kidneys of both control and TCE-treated mice. The identified carbonylated proteins represent skeletal proteins, chaperones, stress proteins, enzymes, plasma protein and proteins involved in signaling pathways. The findings provide a map for further exploring the role of carbonylated proteins in TCE-mediated nephrotoxicity. |
| Related Links | http://europepmc.org/articles/pmc3864976?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864976/pdf https://www.tandfonline.com/doi/pdf/10.3109/15376516.2013.843112 |
| Ending Page | 30 |
| Page Count | 10 |
| Starting Page | 21 |
| ISSN | 15376516 |
| e-ISSN | 15376524 |
| DOI | 10.3109/15376516.2013.843112 |
| Journal | Toxicology mechanisms and methods |
| Issue Number | 1 |
| Volume Number | 24 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2013-10-07 |
| Access Restriction | Open |
| Subject Keyword | Toxicology Autoimmune Nephritis Oxidative Stress Protein Carbonylation Proteomics Trichloroethene |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Toxicology |
