NDLI: Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

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Protocol for the in vitro rearing of honey bee (Apis mellifera L.) workers

Content Provider	Scilit
Author	Schmehl, Daniel R. Tomé, Hudson V. V. Mortensen, Ashley N. Martins, Gustavo Ferreira Ellis, James D.
Copyright Year	2016
Description	The in vitro rearing of worker honey bees (Apis mellifera L.) has become an increasingly important method in honey bee research in general, and in pesticide risk assessment specifically. Authorities from the European Organization for Economic Co-operation and Development and the United States Environmental Protection Agency are requesting data on pesticide impacts on immature bee survivorship prior to registering new crop protection products. Those using the current in vitro rearing protocols have had variable success with immature bee survival and protocol repeatability. Here, we present an improved method for the in vitro rearing of worker honey bees from larvae to adult emergence. We have achieved consistently high survival (>95%) in our control and solvent-control rearing trials. Changes in the proportion of diet components, royal jelly source, maintenance of the developing bee, and rearing environment are the main contributors for our high rearing success and are discussed herein. Our in vitro rearing protocol can be implemented as the standard protocol to determine the impact of pesticides on immature bees because of the protocol's high control survivability, ease in end point determination, and high overall repeatability.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/00218839.2016.1203530?needAccess=true
Ending Page	129
Page Count	17
Starting Page	113
ISSN	00218839
e-ISSN	20786913
DOI	10.1080/00218839.2016.1203530
Journal	Journal of Apicultural Research
Issue Number	2
Volume Number	55
Language	English
Publisher	Informa UK Limited
Publisher Date	2016-03-01
Access Restriction	Open
Subject Keyword	Forestry Honey Bee Apis Mellifera in Vitro Rearing Protocol Artificial Diet Pesticides
Content Type	Text
Resource Type	Article
Subject	Insect Science

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