Proportional subcellular localization of Arabidopsis thaliana RabA1a

Content Provider	Scilit
Author	Kim, Hyeran Kang, Hyangju Kwon, Yun Choi, Jisun Chang, Jeong Ho
Copyright Year	2019
Description	Subcellular localization of trafficking proteins in a single cell affects the assembly of trafficking machinery between organelles and vesicles throughout the targeting pathway. RabGTPase is one of the regulators to direct specific targeting of cargo molecules depending on GDP/GTP bound status. We have recently determined the crystal structures of GDP-bound inactive and both GTP- and GppNHp-bound active forms of Arabidopsis RabA1a. It is notable that the switch regions of RabA1a exhibit conformational changes derived by GDP or GTP binding. However, it was not clear that where the GDP- or GTP-bound RabA1a is localized at the subcellular level in a cell. Here we demonstrate that the distinct proportion of subcellular localization of RabA1a depends on its site-specific mutation as the GDP- or GTP-bound form. RabA1a proteins located at the plasma membrane, endosomes, and cytosol. While the GDP-bound form of $RabA1a^{S27N}$ located more at endosomes than the plasma membrane compared to the proportions of RabA1a wild-type, and the GTP-bound $RabA1a^{Q72L}$ located mainly at the plasma membrane in comparison to RabA1a wild-type and $RabA1a^{S27N}$. These distinct proportional localizations of RabA1a enable a cognate interaction between inactive/active RabA1 and effector molecules to direct specific targeting of its cargo molecules.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/15592324.2019.1581561?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422372/pdf
e-ISSN	15592324
DOI	10.1080/15592324.2019.1581561
Journal	Plant signaling & behavior
Issue Number	3
Volume Number	14
Language	English
Publisher	Informa UK Limited
Publisher Date	2019-02-14
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Raba1a Raba1as27n Raba1aq72l Rabgtpase Subcellular Localization
Content Type	Text