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Oligomeric forms of bacterial malate dehydrogenase: a study of the enzyme from the phototrophic non-sulfur bacterium Rhodovulum steppense A-20s
| Content Provider | Scilit |
|---|---|
| Author | Eprintsev, Alexander T. Falaleeva, Marina I. Lyashchenko, Maya S. Toropygin, Ilya Y. Igamberdiev, Abir U. |
| Copyright Year | 2018 |
| Description | Malate dehydrogenase (EC 1.1.1.37) was purified to homogeneity from the phototrophic purple non-sulfur bacterium Rhodovulum steppense A-20s. According to gel-chromatography and electrophoretic studies, malate dehydrogenase is present as a dimer, tetramer and octamer depending on cultivation conditions. In phototrophic aerobic conditions only the tetrameric form was present, in chemotrophic aerobic conditions all three forms were detected, while in the absence of oxygen the octameric form disappeared. The malate dehydrogenase oligomers are encoded by a single gene and composed of the same 35 kDa polypeptide but differ in pH and temperature optimum, in affinities to malate, oxaloacetate, NADH and $NAD^{+}$ and in regulation by cations and citrate. By modulating the cultivation conditions, it has been established that the dimer participates in the glyoxylate cycle; the tetramer operates in the tricarboxylic acid cycle, and the octamer may be involved in the adaptation to oxidative stress. CXCL10 as a novel exercise-reducible myokine. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/09168451.2017.1411776?needAccess=true |
| Ending Page | 89 |
| Page Count | 9 |
| Starting Page | 81 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/09168451.2017.1411776 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 1 |
| Volume Number | 82 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2018-01-02 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Malate Dehydrogenase Purple Non-sulfur Bacteria Rhodovulum Steppense Tricarboxylic Acid Cycle Glyoxylate Cycle Mdh: Malate Dehydrogenase Oaa: Oxaloacetate Sod: Superoxide Dismutase Tca Cycle: Tricarboxylic Acid Cycle |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
