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Investigation of mechanisms of mesenchymal stem cells for treatment of diabetic nephropathy via construction of a miRNA-TF-mRNA network
| Content Provider | Scilit |
|---|---|
| Author | Yang, Hailing Zhang, Xiaofei Xin, Guangda |
| Copyright Year | 2018 |
| Description | Background: Recent studies have reported that mesenchymal stem cells (MSCs) exert therapeutic effects on the treatment of diabetic nephropathy (DN), but the underlying mechanisms remain unclear. Methods: A dataset GSE65561 was obtained from Gene Expression Omnibus (GEO) database, which contained four healthy control samples (group 1), four healthy controls samples co-cultured with MSCs (group 2), five DN samples (group 3) and five DN samples co-cultured with MSCs (group 4). The differentially expressed genes (DEGs) between group 3 vs. group 1 and group 4 vs. group 2 were constructed using Linear Models for Microarray (LIMMA) package package. Then, DAVID was used to analyze the functional enrichment of DEGs. Based on STRING database the protein-protein interaction (PPI) network was visualized by the Cytoscape plug-in CytoNCA. Besides, the hub miRNAs and transcription factors (TFs) regulating DEGs were predicted using Webgestalt. Results: Totally, 303 up-regulated and 88 down-regulated DEGs were shared in group 3 vs. group 1 and group 4 vs. group 2. Besides, the up-regulated DEGs were mainly enriched in 'translation' and 'translational elongation', while the down-regulated genes were only enriched in 'protein kinase activity'. RPS27A and RPLP0 had a higher degree in the PPI network and they were regulated by EIF3M. In addition, ETF1 was predicted to be an important gene, which was regulated by miR-150, miR-134 and EIF2S1. Conclusions: RPS27A, RPLP0 and ETF1 may be potential targets for MSCs on the treatment of DN. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/0886022X.2017.1421556?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6014302/pdf |
| Ending Page | 145 |
| Page Count | 10 |
| Starting Page | 136 |
| ISSN | 0886022X |
| e-ISSN | 15256049 |
| DOI | 10.1080/0886022x.2017.1421556 |
| Journal | Renal Failure |
| Issue Number | 1 |
| Volume Number | 40 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2018-03-13 |
| Access Restriction | Open |
| Subject Keyword | Cell Tissue Engineering Diabetic Nephropathy Differentially Expressed Gene Protein-protein Interaction Network Microrna Transcription Factor |
| Content Type | Text |
| Resource Type | Article |
| Subject | Critical Care and Intensive Care Medicine Nephrology |
