Growth and sporulation of Phytophthora ramorum in vitro in response to temperature and light

Content Provider	Scilit
Author	Englander, Larry Browning, Marsha Tooley, Paul W.
Copyright Year	2006
Description	Phytophthora ramorum, recently found in the US, is causing concern for hardwood forests and the nursery industry. In an effort to identify some of the environmental limitations to growth and sporulation we undertook a laboratory study of four US and three European (EU) isolates. On V8 media, isolates grew when incubated at 2–28 C and produced chlamydospores at 8–28 C. Sporangia were produced at all temperatures tested: 10–30 C for US isolates and 6–26 C for EU isolates. Optimal temperatures were 16–26 C for growth, 14–26 C for chlamydospore production and 16–22 C for sporangia production. US isolates grew less and produced fewer spores when exposed to increasing doses of near-UV radiation (50–300 $μW/cm^{2}$) and visible radiation (250–1500 $μW/cm^{2}$). EU isolates were exposed to 300 $μW/cm^{2}$ near-UV only, which significantly reduced growth of one of three isolates and had no significant effect on spore production. In our studies P. ramorum tolerated a broad range of temperature and light conditions, which suggests that it is capable of establishment in a wide geographic area.
Related Links	http://www.mycologia.org/content/98/3/365.full.pdf
Ending Page	373
Page Count	9
Starting Page	365
ISSN	00275514
e-ISSN	15572536
DOI	10.3852/mycologia.98.3.365
Journal	Mycologia
Issue Number	3
Volume Number	98
Language	English
Publisher	Informa UK Limited
Publisher Date	2006-05-01
Access Restriction	Open
Subject Keyword	Mycology Optimal Phytophthora Temperatures Chlamydospores Tolerated Ramorum Sporangia Μw/cm2
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Physiology Plant Science Medicine Molecular Biology Ecology, Evolution, Behavior and Systematics