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RETRACTED ARTICLE: The protective role of microRNA-140-5p in synovial injury of rats with knee osteoarthritis via inactivating the TLR4/Myd88/NF-κB signaling pathway
| Content Provider | Scilit |
|---|---|
| Author | Huang, Xiaoqiang Qiao, Feng Xue, Peng |
| Copyright Year | 2019 |
| Description | Recently, many studies have revealed the effect of microRNAs (miRNAs) in knee osteoarthritis (KOA). This study aims to explore the role of miR-140-5p in protective effects and mechanisms of synovial injury of rats with KOA via regulating the TLR4/Myd88/NF-κB signaling pathway. The models of KOA Wistar rats were established by operation of anterior cruciate ligament transection. Rats were injected with agomir NC or miR-140-5p agomir. MiR-140-5p expression in KOA synovial tissues and synoviocytes was evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The synoviocytes were transfected with mimics NC sequence and miR-140-5p mimics sequence. The expression of TLR4/Myd88/NF-κB signaling pathway-related proteins was measured by RT-qPCR and western blot analysis. The proliferation and apoptosis of synoviocytes in rats with KOA were evaluated by a string of experiments. The expression levels of inflammatory factors in KOA synovial tissues and synoviocytes were detected. MiR-140-5p was down-regulated in KOA synovial tissues and synoviocytes. Upregulation of miR-140-5p could inhibit the inflammation reaction and the apoptosis of synoviocytes as well as promote proliferation of synoviocytes of rats with KOA. Furthermore, upregulated miR-140-5p could inactivate the TLR4/Myd88/NF-κB signaling pathway in rats with KOA. This study suggests that upregulated miR-140-5p could protect synovial injury by restraining inflammation reaction and apoptosis of synoviocytes in KOA rats via TLR4/Myd88/NF-κB signaling pathway inactivation. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6738526/pdf |
| Ending Page | 2358 |
| Page Count | 15 |
| Starting Page | 2344 |
| ISSN | 15384101 |
| e-ISSN | 15514005 |
| DOI | 10.1080/15384101.2019.1647025 |
| Journal | Cell Cycle |
| Issue Number | 18 |
| Volume Number | 18 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-07-29 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Microrna Tlr4/myd88/nf Κb Signaling Pathway Knee Osteoarthritis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine Molecular Biology |
