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Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
| Content Provider | Scilit |
|---|---|
| Author | Zhao, Yan Zhang, Qiang Meng, Qingfeng Wu, Fenglian Zhang, Lihua Tang, Yao Guan, Yuanyuan An, Lixin |
| Copyright Year | 2017 |
| Description | Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite Yan Zhao,1,* Qiang Zhang,2,* Qingfeng Meng,3 Fenglian Wu,4 Lihua Zhang,1 Yao Tang,1 Yuanyuan Guan,1 Lixin An1 1Department of Allergy, The First Affiliated Hospital, Harbin Medical University, Harbin, 2Department of Otorhinolaryngology Head and Neck Surgery, The Affiliated Yantai Yuhuangding Hospital, Qingdao University, Yantai, 3Department of Ophthalmology, The First Affiliated Hospital, Harbin Medical University, Harbin, 4Department of Burn and Plastic Surgery, The First Affiliated Hospital of Qinhuangdao, Qinhuangdao, People's Republic of China *These authors contributed equally to this work Semiquantitative and rapid detection of specific IgE (sIgE) with well clinical relevance to house dust mite (HDM) are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. However, the conventional IgE measurement systems are time-consuming, complicated and require special instruments. Herein, we overcome the above limitations of sIgE to HDM detection system by developing a quantum dot nanobeads-based lateral flow immunoassay and an image analysis procedure. The proposed detection system could semiquantitatively measure the IgE in a linear range of 0.2–10 U/mL. Moreover, there is a well correlation between the developed detection system and the clinical symptoms by a comparison study using 56 positive patients' sera and 40 healthy control sera. The proposed detection system is simple, robust and easy-to-use and promising for in home test. Keywords: lateral flow immunoassay, quantum dots, house dust mite, IgE |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503493/pdf https://www.dovepress.com/getfile.php?fileID=37252 |
| Ending Page | 4812 |
| Page Count | 8 |
| Starting Page | 4805 |
| e-ISSN | 11782013 |
| DOI | 10.2147/ijn.s134539 |
| Journal | International Journal of Nanomedicine |
| Volume Number | 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2017-07-01 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Instrumentation House Dust Mite Lateral Flow Immunoassay Quantum Dots |
| Content Type | Text |
| Resource Type | Article |
