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Production and Characterization of Murine Single Chain Fv Antibodies to Aflatoxin B 1 Derived From a Pre-immunized Antibody Phage Display Library System
| Content Provider | Scilit |
|---|---|
| Author | O'kennedy, Richard Daly, Stephen Dillon, Paul Manning, Bernadette Dunne, Lynsey Killard, Anthony |
| Copyright Year | 2002 |
| Description | The production and characterization of recombinant antibodies to aflatoxin B 1 (AFB 1 ), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B 1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB 1 , in this format, with the assays being sensitive and reproducible. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/0954010021000096373?needAccess=true https://core.ac.uk/reader/11307947 |
| Ending Page | 274 |
| Page Count | 20 |
| Starting Page | 255 |
| ISSN | 09540105 |
| e-ISSN | 14653443 |
| DOI | 10.1080/0954010021000096373 |
| Journal | Food and Agricultural Immunology |
| Issue Number | 4 |
| Volume Number | 14 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2002-12-01 |
| Access Restriction | Open |
| Subject Keyword | Analytical Chemistry Aflatoxin B 1 Single Chain Fv Phage Display Technology Surface Plasmon Resonance |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology Food Science Agronomy and Crop Science |
