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Real-time quaking-induced conversion
| Content Provider | Scilit |
|---|---|
| Author | Atarashi, Ryuichiro Sano, Kazunori Satoh, Katsuya Nishida, Noriyuki |
| Copyright Year | 2011 |
| Description | We recently developed a new in vitro amplification technology, designated “real-time quaking-induced conversion (RT-QUIC)”, for detection of the abnormal form of prion protein (PrPSc) in easily accessible specimens such as cerebrospinal fluid (CSF). After assessment of more than 200 CSF specimens from Japanese and Australian patients, we found no instance of a false positive, and more than 80% accuracy for the correct diagnosis of sporadic Creutzfeldt–Jakob disease (sCJD). Furthermore, the RT-QUIC can be applied to other prion diseases, including scrapie, chronic wasting disease (CWD), and bovine spongiform encephalopathy (BSE), and is able to quantify prion seeding activity when combined with an end-point dilution of samples. These results indicate that the RT-QUIC, with its high sensitivity and specificity, will be of great use as an early, rapid and specific assay for prion diseases. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/pri.5.3.16893?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226039/pdf |
| Ending Page | 153 |
| Page Count | 4 |
| Starting Page | 150 |
| ISSN | 19336896 |
| e-ISSN | 1933690X |
| DOI | 10.4161/pri.5.3.16893 |
| Journal | Prion |
| Issue Number | 3 |
| Volume Number | 5 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2011-07-01 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Virology Rt-quic Real-time Quaking-induced Conversion Creutzfeldt-jakob Disease Cerebrospinal Fluid |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Infectious Diseases Biochemistry Cellular and Molecular Neuroscience |
