NDLI: ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions

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ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions

Content Provider	Scilit
Author	Edelmann, Franziska Niedner, Annika Niessing, Dierk
Copyright Year	2015
Description	Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/15476286.2015.1017217?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615642/pdf
Ending Page	237
Page Count	5
Starting Page	233
ISSN	15476286
e-ISSN	15558584
DOI	10.1080/15476286.2015.1017217
Journal	RNA Biology
Issue Number	3
Volume Number	12
Language	English
Publisher	Informa UK Limited
Publisher Date	2015-03-04
Access Restriction	Open
Subject Keyword	Cell Biology Ash1 Mrna Budding Yeast in Vitro Reconstitution Myosin Macromolecular Assembly Rna Localization
Content Type	Text
Subject	Cell Biology Molecular Biology

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