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Suppression Of Hepatitis C Virus Core Protein By Short Hairpin Rna Expression Vectors In The Core Protein Expression Huh-7 Cells
| Content Provider | Scilit |
|---|---|
| Author | Suzuki, Hitoshi Kaneko, Hiroyasu Tamai, Nobushige Shimotohno, Kunitada Miyano-Kurosaki, Naoko Takaku, Hiroshi |
| Copyright Year | 2007 |
| Description | Short hairpin RNAs (shRNAs) efficiently inhibit gene expression by RNA interference. Here, we report the efficient inhibition by DNA-based vector-derived shRNAs of core protein expression in Huh-7 cells. The shRNAs were designed to target the core region of the hepatitis C virus (HCV) genome. The core region is the most conserved region in the HCV genome, making it an ideal target for shRNAs. We identified an effective site on the core region for suppression of the HCV core protein. The HCV core protein in core protein-expressing Huh-7 cells was downregulated by core protein-shRNA expression vectors (core-shRNA-452, 479, and 503). Our results support the feasibility of using shRNA-based gene therapy to inhibit HCV core protein production. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2403745/pdf |
| Ending Page | 820 |
| Page Count | 6 |
| Starting Page | 815 |
| ISSN | 15257770 |
| e-ISSN | 15322335 |
| DOI | 10.1080/15257770701503787 |
| Journal | Nucleosides, nucleotides & nucleic acids |
| Issue Number | 6-7 |
| Volume Number | 26 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2007-11-26 |
| Access Restriction | Open |
| Subject Keyword | Journal: Nucleosides, Nucleotides & Nucleic Acids Infectious Diseases Rnai Short Hairpin Rnas (shrnas) Hepatitis C Virus (hcv) Core Protein Huh-7 Cells Gfp Dsred |
| Content Type | Text |
| Resource Type | Article |
| Subject | Molecular Medicine Genetics Biochemistry Medicine |
