NDLI: Cell Separation Using Flow Cytometric Cell Sorting

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Cell Separation Using Flow Cytometric Cell Sorting

Content Provider	Scilit
Author	Recktenwald, Diether
Copyright Year	1997
Description	I. INTRODUCTION Flow cytometric cell sorting rapidly separates cells one at a time based primarily on optical and fluorescent properties. Fluorescent dyes to specific cellular targets or antibodies (Abs) tagged with fluorochromes are used to identify particular cells. Cells in liquid suspension flow past a sensing zone and are analyzed for the optical signal they emit. Analysis rates can be greater than 10,000 cells/s. Cells whose optical characteristics fall within preselected ranges or “gates” generate an electronic signal that initiates sorting of the cell. Less than one millisecond after a wanted cell is analyzed, it can be physically separated. Some early researchers coined the acronym FACS (fluorescenceactivated cell sorting) for this technique, and FACS™ is currently a trademark of Becton Dickinson Immunocytometry Systems. Since characteristics other than fluorescence can be used to analyze the cells, this chapter will use a more general term, flow sorting, for this technique. Book Name: Cell Separation Methods and Applications
Related Links	https://content.taylorfrancis.com/books/download?dac=C2006-0-18656-4&isbn=9780429153549&doi=10.1201/9781482273649-37&format=pdf
Ending Page	277
Page Count	21
Starting Page	257
DOI	10.1201/9781482273649-37
Language	English
Publisher	Informa UK Limited
Publisher Date	1997-11-04
Access Restriction	Open
Subject Keyword	Book Name: Cell Separation Methods and Applications Optical Antibodies Fluorescence Facs Cell Sorting Flow Cytometric
Content Type	Text
Resource Type	Chapter

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