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γ-H2AX expression detected by immunohistochemistry correlates with prognosis in early operable non-small cell lung cancer
| Content Provider | Scilit |
|---|---|
| Author | Matthaios, Dimitrios Foukas, Periklis G. Hountis, Panagiotis Trypsianis, Grigorios Panayiotides, Ioannis G. Chatzaki, Ekaterini Bouros, Demosthenes Karakitsos, Petros Kakolyris, Stylianos Kefala, Maria Pantelidaki, Ekaterini |
| Copyright Year | 2012 |
| Abstract | Background: Phosphorylation of the H2AX histone is an early indicator of DNA double-strand breaks and of the resulting DNA damage response. In the present study, we assessed the expression and prognostic significance of γ-H2AX in a cohort of 96 patients with operable non-small cell lung carcinoma. Methods: Ninety-six paraffin-embedded specimens of non-small cell lung cancer patients were examined. All patients underwent radical thoracic surgery of primary tumor (lobectomy or pneumonectomy) and regional lymph node dissection. γ-H2AX expression was assessed by standard immunohistochemistry. Follow-up was available for all patients; mean duration of follow-up was 27.50 ± 14.07 months (range 0.2–57 months, median 24 months). Results: Sixty-three patients (65.2%) died during the follow-up period. The mean survival time was 32.2 ± 1.9 months (95% confidence interval [CI]: 28.5–35.8 months; median 30.0 months); 1-, 2- and 3-year survival rates were 86.5% ± 3.5%, 57.3% ± 5.1%, and 37.1% ± 5.4%, respectively. Low γ-H2AX expression was associated with a significantly better survival as compared with those having high γ-H2AX expression (35.3 months for low γ-H2AX expression versus 23.2 months for high γ-H2AX expression, P = 0.009; hazard ratio [HR] 1.95, 95% CI: 1.15–3.30). Further investigation with multivariate Cox proportional hazards regression analysis revealed that high expression of γ-H2AX remained an independent prognostic factor of shorter overall survival (HR 2.15, 95% CI: 1.22–3.79, P = 0.026). A combined p53/ γ-H2AX analysis was performed, and we found that the p53 low/γ-H2AX low phenotype was associated with significantly better survival compared with all other phenotypes. Conclusion: Our study is the first to demonstrate that expression of γ-H2AX detected by immunohistochemistry may represent an independent prognostic indicator of overall survival in patients with non-small cell lung cancer. Further studies are needed to confirm our results. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3501397/pdf https://www.dovepress.com/getfile.php?fileID=14353 |
| Ending Page | 314 |
| Page Count | 6 |
| Starting Page | 309 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s36995 |
| Journal | OncoTargets and therapy |
| Volume Number | 5 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2012-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Pathology H2ax Dna Damage Response Non-small Cell Lung Cancer P53 Prognosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
