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Topologically Associating Domains: An invariant framework or a dynamic scaffold?
| Content Provider | Scilit |
|---|---|
| Author | Potts, Caelin Corces, Victor G. |
| Copyright Year | 2015 |
| Description | Metazoan genomes are organized into regions of topologically associating domains (TADs). TADs are demarcated by border elements, which are enriched for active genes and high occupancy architectural protein binding sites. We recently demonstrated that 3D chromatin architecture is dynamic in response to heat shock, a physiological stress that downregulates transcription and causes a global redistribution of architectural proteins. We utilized a quantitative measure of border strength after heat shock, transcriptional inhibition, and architectural protein knockdown to demonstrate that changes in both transcription and architectural protein occupancy contribute to heat shock-induced TAD dynamics. Notably, architectural proteins appear to play a more important role in altering 3D chromatin architecture. Here, we discuss the implications of our findings on previous studies evaluating the dynamics of TAD structure during cellular differentiation. We propose that the subset of variable TADs observed after differentiation are representative of cell-type specific gene expression and are biologically significant. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/19491034.2015.1096467?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915497/pdf |
| Ending Page | 434 |
| Page Count | 5 |
| Starting Page | 430 |
| ISSN | 19491034 |
| e-ISSN | 19491042 |
| DOI | 10.1080/19491034.2015.1096467 |
| Journal | Nucleus |
| Issue Number | 6 |
| Volume Number | 6 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2015-11-02 |
| Access Restriction | Open |
| Subject Keyword | Journal: Nucleus Biophysics Cell Biology Architectural Proteins Chromatin Ctcf Differentiation Epigenetics Heat Shock Insulators Tad 3d Architecture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
