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Neutrophil Gelatinase-Associated Lipocalin Protects Renal Tubular Epithelial Cell in Ischemic/Reperfusion Injury Rats via Apoptosis-Regulating Proteins
| Content Provider | Scilit |
|---|---|
| Author | Gong, Li Yu, Hua Zhuge, Yifeng Yu, Qing |
| Copyright Year | 2012 |
| Description | We investigated the role of neutrophil gelatinase-associated lipocalin (NGAL) on renal tubular epithelial cell in the renal ischemia/reperfusion injury (IRI) rats. Male Sprague–Dawley rats were randomly assigned to three groups. The control group (n = 5) underwent left nephrectomy. The ischemia/reperfusion (I/R) + normal saline (NS) (n = 5) and I/R + NGAL groups (n = 5) were subjected to 45 min right renal ischemia followed by 48 h of reperfusion after left nephrectomy. The pathological changes of kidney tissues were investigated using hematoxylin–eosin staining; renal tubular epithelial cell apoptosis was detected using terminal dUTP nick-labeling method; expression of apoptosis-regulating protein Fas and Bcl-2 was measured using real-time polymerase chain reaction, Western blot, and immunohistochemical staining. Compared with I/R + NS group, kidney tissues from I/R + NGAL group revealed reduced histological damage and a decreased number of renal tubular epithelial cell apoptosis (9.2 ± 2.53 nuclei or 4.0 ± 0.7 per high-power field vs. 20.3 ± 3.7 nuclei or 8.1 ± 0.3 per high-power field); rats with NGAL showed downregulated fas mRNA (2.34 ± 0.51 vs. 6.84 ± 2.34), fas protein (0.65 ± 0.05 vs. 0.95 ± 0.08), and upregulated bcl-2 protein (0.33 ± 0.05 vs. 0.24 ± 0.03). The results had statistical significance (p < 0.05). We think NGAL could protect against renal IRI and might be related to decreasing tubular epithelial cell apoptosis via adjusting the expression of apoptosis-regulating proteins. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/0886022X.2012.678173?needAccess=true |
| Ending Page | 783 |
| Page Count | 7 |
| Starting Page | 777 |
| ISSN | 0886022X |
| e-ISSN | 15256049 |
| DOI | 10.3109/0886022x.2012.678173 |
| Journal | Renal Failure |
| Issue Number | 6 |
| Volume Number | 34 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2012-04-10 |
| Access Restriction | Open |
| Subject Keyword | Journal: Renal Failure Ngal Renal Tubular Epithelial Cells Ischemia/reperfusion Injury Cell Apoptosis Apoptosis-regulating Protein |
| Content Type | Text |
| Resource Type | Article |
| Subject | Critical Care and Intensive Care Medicine Nephrology |
