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Effects on biological properties of embryo‐adapted beaudette strain of infectious bronchitis virus of passage through chick embryo trachea organ culture
| Content Provider | Scilit |
|---|---|
| Author | Yachida, S. Aoyama, A. Takahashi, N. Iritani, Y. Katagiri, K. |
| Copyright Year | 1979 |
| Description | Two substrains derived from an uncloned 71st embryo $(E_{7)}$) passage and a cloned 71st embryo‐10th chick embryo kidney cell $(E_{71}CEK_{10}$) passage of the Beaudette strain of avian infectious bronchitis virus were back‐passaged through organ cultures of chick embryo trachea. The organ culture‐passaged viruses had altered biological properties such as absence of or delayed lethality for chick embryos and lack or diminution of plaque‐forming ability in chick embryo kidney monolayers but they showed no clear change of pathogenicity for 3‐week‐old chickens. These viruses regained their lethality for chick embryos, their cytopathic effects, and their plaque‐forming ability in chick embryo kidney cells after they had been back‐passaged through chick embryos, depending on the passage number. The results of cross neutralisation tests using the constant serum‐variable virus method in chick embryos indicated little antigenic difference between the original virus and various host‐passaged derivative strains. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.1080/03079457908418359?needAccess=true |
| Ending Page | 324 |
| Page Count | 12 |
| Starting Page | 313 |
| ISSN | 03079457 |
| e-ISSN | 14653338 |
| DOI | 10.1080/03079457908418359 |
| Journal | Avian Pathology |
| Issue Number | 4 |
| Volume Number | 8 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1979-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Avian Pathology Virology Chick Embryo Embryos Organ Culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Animal Science and Zoology Food Animals |
