Intervention des lectines fongiques dans les événements précoces de reconnaissance arbre/champignon au cours de la formation des ectomycorhizes

Content Provider	Scilit
Author	Guillot, Jean Giollant, Michel Damez, Mireille Dusser, Martine
Copyright Year	1994
Description	The occurence of lectin activity in a large number of higher fungi and the existence of an often high degree of specificity in the associations of fungi with trees prompted us to determine whether the fungal lectins might be involved in the recognition of the symbionts. To this end, we chose as a model the milk cap mushrooms of the Dapetes group, all of which are associated with conifers, but which exhibit a remarkable specificity. Thus, the morphologically very similar Lactarius deliciosus, Lactarius deterrimus, and Lactarius salmonicolor are associated with the pine, the spruce, and the fir, respectively. In order to back up the lectinic hypothesis, we have confirm three essential points: 1) The lectins isolated from the three milk cap mushrooms are different in structure and specificity. Although their molecular weight are very close, lectins have different subunits, isoelectric point and amino acid composition. All the lectins are specifically inhibited by the disaccharide D-Galß1 → 3D-GalNAc, but with very different intensities in relation with non identical accurate specificities. 2) Previously isolated from the carpophores, lectins are also expressed at the surface of the hyphae. A lectin activity was detected in the in vitro culture medium, and a lectin was released by enzyme action from the wall of the mycelial hyphae. Anti-lectin antibodies reveal, by immunofluorescence and electronic microscopy, the presence of a specific lectin in the wall of mycelium. 3) By the mean of immunofluorescence technique, receptor sites for the lectin from the specifically associated mushroom were found on the particular cells of the roots, for coniferous seedlings (Pinus sylvestris, Picea abies, Abies alba).
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/12538078.1994.10515181?needAccess=true
Ending Page	447
Page Count	5
Starting Page	443
ISSN	12538078
e-ISSN	21663408
DOI	10.1080/12538078.1994.10515181
Journal	Acta Botanica Gallica
Issue Number	4
Volume Number	141
Language	English
Publisher	Informa UK Limited
Publisher Date	1994-01-01
Access Restriction	Open
Subject Keyword	Journal: Acta Botanica Gallica Mycology Mycorrhiza Lectins Mushrooms Specific Recognition Cell Wall
Content Type	Text
Resource Type	Article
Subject	Plant Science