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Oriented Embedding of Biological Materials and Accurate Localization for Ultrathin Sectioning
| Content Provider | Scilit |
|---|---|
| Author | Gorycki, Michael A. |
| Copyright Year | 1966 |
| Description | Gelatin capsules with rounded ends clipped off and open ends moistened, affixed to a glass slide and sealed with a 15% gelatin solution are used to embed blocks of tissue in plastic. The surface of the slide serves as an orientation plane for structures of the tissue. The plane end of capsules of polymerized plastic containing no tissue is used in embedding frozen tissue sections. The plastic-infiltrated section is flattened against the capsule end under the weight of a 3/4 inch square of plate glass so that larger sections may be cut and surveyed. Embedding cultured cell monolayers grown on coverslips is accomplished in a comparable manner, but the square of plate glass is not needed as a weight. Block-face localization methods depend on the type of material embedded. With blocks of tissue it is achieved by moistening the face with xylene to develop relief. Thin tissue sections are examined by transmitted light, while cell monolayers are stained on the capsule end with methylene blue. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/10520296609116277 |
| Ending Page | 42 |
| Page Count | 6 |
| Starting Page | 37 |
| ISSN | 00389153 |
| DOI | 10.3109/10520296609116277 |
| Journal | Stain technology |
| Issue Number | 1 |
| Volume Number | 41 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1966-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Stain Technology Anesthesiology Accurate Localization Oriented Embedding Ultrathin Sectioning Biological Materials Embedding of Biological Materials and Accurate Localization for Ultrathin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Anatomy |
