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Characterization of the T-dependent antibody response (TDAR) to keyhole limpet hemocyanin (KLH) in the Göttingen minipig
| Content Provider | Scilit |
|---|---|
| Author | Peachee, Vanessa L. Smith, Matthew J. Beck, Melissa J. Stump, Donald G. White, Kimber L. |
| Copyright Year | 2013 |
| Description | Recently, there has been a renewed interest in the use of the minipig as an alternative to dogs and non-human primates for conducting toxicological assessments in non-rodent species. Since the T-dependent antibody response (TDAR) is one of the most widely-accepted assays used in the assessment of immunocompetence, the present study was undertaken to characterize the primary and secondary TDAR to keyhole limpet hemocyanin (KLH) in the Göttingen Minipig(®). Following primary immunization with either 2 or 10 mg KLH, anti-swine IgM and IgG ELISAs were optimized and individual animal responses were evaluated over time. Immunization with 10 mg KLH on Day 0 promoted primary IgM responses that peaked 6-9 days after antigen administration, while primary IgG levels peaked on Day 13 or 14. Secondary IgG antibody levels (following secondary injection with 2 mg KLH on Day 14) plateaued on Days 20-22. Anti-KLH antibody levels were decreased in minipigs treated with cyclophosphamide (CPS), a known immunosuppressant, at doses ranging from 12.5-50 mg/kg/day, while antibody levels in animals treated with 2.5 mg CPS/kg/day were similar to levels in saline-treated swine. These results demonstrate that the Göttingen Minipig(®) can be a useful alternative non-rodent species to the dog and the non-human primate for evaluating the TDAR to KLH in regulatory assessments of immunotoxicity. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/1547691X.2013.853716?needAccess=true |
| Ending Page | 382 |
| Page Count | 7 |
| Starting Page | 376 |
| ISSN | 1547691X |
| e-ISSN | 15476901 |
| DOI | 10.3109/1547691x.2013.853716 |
| Journal | Journal of immunotoxicology |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2013-11-12 |
| Access Restriction | Open |
| Subject Keyword | Journal: Journal of Immunotoxicology Toxicology Immunotoxicity Tdar Keyhole Limpet Hemocyanin Miniature Swine Minipig |
| Content Type | Text |
| Resource Type | Article |
| Subject | Toxicology Immunology |
