NDLI: 14C-Labeled Lignins as Substrates for the Study of Lignin Biodegradation and Transformation

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14C-Labeled Lignins as Substrates for the Study of Lignin Biodegradation and Transformation

Content Provider	Scilit
Author	Crawford, Ronald L. Robinson, Larry E. Cheh, Albert M.
Copyright Year	2018
Description	The wide applicability of$ ^{14}$C-based lignin biodegradation assays to lignin-related research problems is obvious. The importance of bacterial groups in processes of lignin degradation and transformation must be reevaluated in light of studies of bacterial decomposition of$ ^{14}$C-based lignins. Radioisotopic biodegradation assays should become valuable tools to microbial ecologists who wish to examine activities of natural populations of lignin-decomposing microorganisms in ecosystems such as the forest floor. Probably the most useful of nonisotopic techniques for following lignin biodegradation in natural lignocelluloses has been the so-called soil block procedure, which is still in use in many laboratories. Substrates such as cellulose or glucose served as cosubstrates for lignin degradation. Lignin is biosynthesized in vascular plants by a branching sequence of reactions, beginning with $CO_{2}$ and $H_{2}$O and proceeding by way of the aromatic amino acid, phenylalanine. Incorporation of$ ^{14}$C-phenylalanine into plant proteins poses somewhat more of a problem than incorporation into polysaccharides. Book Name: Lignin Biodegradation: Microbiology, Chemistry, and Potential Applications
Related Links	https://api.taylorfrancis.com/content/chapters/edit/download?identifierName=doi&identifierValue=10.1201/9781351074063-3&type=chapterpdf
Ending Page	76
Page Count	15
Starting Page	62
DOI	10.1201/9781351074063-3
Language	English
Publisher	Informa UK Limited
Publisher Date	2018-01-18
Access Restriction	Open
Subject Keyword	Book Name: Lignin Biodegradation: Microbiology, Chemistry, and Potential Applications Characterization and Testing of Materials Microorganisms Substrates Lignin Biodegradation Phenylalanine Proteins Biodegradation Assays Based Lignin
Content Type	Text
Resource Type	Chapter

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