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Regulatory insights into the production of UDP-N-acetylglucosamine by Lactobacillus casei

Content Provider	Scilit
Author	Rodríguez-Díaz, Jesús Rubio-Del-Campo, Antonio Yebra, María J.
Copyright Year	2012
Description	UDP-N-acetylglucosamine (UDP-GlcNAc) is an important sugar nucleotide used as a precursor of cell wall components in bacteria, and as a substrate in the synthesis of oligosaccharides in eukaryotes. In bacteria UDP-GlcNAc is synthesized from the glycolytic intermediate D-fructose-6-phosphate (fructose-6P) by four successive reactions catalyzed by three enzymes: glucosamine-6-phosphate synthase (GlmS), phosphoglucosamine mutase (GlmM) and the bi-functional enzyme glucosamine-1-phosphate acetyltransferase/ N-acetylglucosamine-1-phosphate uridyltransferase (GlmU). We have previously reported a metabolic engineering strategy in Lactobacillus casei directed to increase the intracellular levels of UDP-GlcNAc by homologous overexpression of the genes glmS, glmM and glmU. One of the most remarkable features regarding the production of UDP-GlcNAc in L. casei was to find multiple regulation points on its biosynthetic pathway: (1) regulation by the NagB enzyme, (2) glmS RNA specific degradation through the possible participation of a glmS riboswitch mechanism, (3) regulation of the GlmU activity probably by end product inhibition and (4) transcription of glmU.
Related Links	http://www.tandfonline.com/doi/pdf/10.4161/bioe.21271?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3489710/pdf https://www.landesbioscience.com/journals/bioe/2012BIOBUGS0245R.pdf
Ending Page	342
Page Count	4
Starting Page	339
ISSN	21655979
e-ISSN	21655987
DOI	10.4161/bioe.21271
Journal	Bioengineered
Issue Number	6
Volume Number	3
Language	English
Publisher	Informa UK Limited
Publisher Date	2012-11-24
Access Restriction	Open
Subject Keyword	Journal: Bioengineered Biotechnology and Microbiology Udp-n-acetylglucosamine Lactobacillus Glucosamine-6-phosphate Synthase Phosphoglucosamine Mutase Glucosamine-1-phosphate Acetyltransferase N-acetylglucosamine-1-phosphate Uridyltransferase
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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