Late Expression of Tumor Necrosis Factor-α IS Markedly Depressed in Patients with IGA Nephropathy

Content Provider	Scilit
Author	Bull, Robert L. Julian, Bruce A. Jackson, Susan
Copyright Year	1998
Description	In this study we demonstrate that peripheral blood mononuclear cells (PBMC) of patients with IgA nephropathy (IgAN) express mRNA for tumor necrosis factor alpha (TNF-α) at a significantly lower frequency after stimulation for 24 hours with pokeweed mitogen than do PBMC of a control population. These differences were not seen in patient samples stimulated for only 3 hours. To identify the cells responsible for TNF-α expression after overnight mitogen stimulation, we performed reverse transcriptase polymerase chain reaction analysis after preparative flow cytometry of PBMC labelled with fluorescent monoclonal antibodies. After stimulation for 24 hours, PBMC of all patients tested displayed no detectable TNF-α mRNA; in the control PBMC, TNF-α mRNA was present in CD4+ and CD8+ T cells, as well as in CD19+ B cells in some samples. These results suggest that diminished expression of TNF-α by T cells may play a role in the pathogenesis of IgAN.
Related Links	http://www.tandfonline.com/doi/pdf/10.3109/08820139809070898
Ending Page	255
Page Count	13
Starting Page	243
ISSN	08820139
e-ISSN	15324311
DOI	10.3109/08820139809070898
Journal	Immunological Investigations
Issue Number	4-5
Volume Number	27
Language	English
Publisher	Informa UK Limited
Publisher Date	1998-01-01
Access Restriction	Open
Subject Keyword	Journal: Immunological Investigations Pathology Urology and Nephrology Antibodies Necrosis Tumor Stimulation Igan Mitogen Tnf Α
Content Type	Text
Resource Type	Article
Subject	Medicine Immunology