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TNRC6C-AS1 Promotes Thyroid Cancer Progression by Upregulating LPAR5 via miR-513c-5p
| Content Provider | Scilit |
|---|---|
| Author | Tong, Chuanming Wang, Chuan Wang, Yajie Xiao, Xiongsheng |
| Copyright Year | 2021 |
| Description | Background: Considering the combined role of long non-coding RNA (lncRNAs)-microRNA (miRNA)-mRNA in tumorigenesis, the purpose of this study was to investigate how TNRC6C-AS1 regulates the expression of lysophosphatidic acid receptor 5 (LPAR5) by modulating miR-513c-5p, thus influencing the progression of thyroid cancer (THCA). Methods: qRT-PCR and Western blotting were performed to detect the expression levels of TNRC6C-AS1, miR-513c-5p, and LPAR5 in THCA tissues and cell lines. The viability, proliferation, migration, and invasion were assessed using CCK-8, BrdU, wound healing, and transwell migration assays, respectively. Dual-luciferase reporter assay, RIP assay, and RNA pull-down assay were used to evaluate the relationship between TNRC6C-AS1, miR-513c-5p, and LPAR5. Results: TNRC6C-AS1 was highly expressed in THCA tissues, and knockout of TNRC6C-AS1 reduced the viability, proliferation, migration, and invasion of THCA cells. TNRC6C-AS1 competitively adsorbed miR-513c-5p. In addition, the biological function of TNRC6C-AS1 was blocked by knocking down the thyroid cell line TNRC6C-AS1 with miR-513c-5p inhibitor transfection. LPAR5 is the target gene for miR-513c-5p, which has the ability to eliminate the influence of miR-513c-5p on THCA cells. Conclusion: The TNRC6C-AS1/miR-513c-5p/LPAR5 axis is a novel signaling pathway that modulates THCA progression and may be a potential target for cancer therapy. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=72344 |
| Ending Page | 6155 |
| Page Count | 15 |
| Starting Page | 6141 |
| ISSN | 11791322 |
| DOI | 10.2147/cmar.s312621 |
| Journal | Cancer Management and Research |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2021-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Management and Research Oncology Tnrc6c-as1 Thca Proliferation Migration Viability |
| Content Type | Text |
| Resource Type | Article |
| Subject | Oncology |
