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Human bone marrow mesenchymal stem cells expressing SDF-1 promote hematopoietic stem cell function of human mobilised peripheral blood CD34+cells in vivo and in vitro
| Content Provider | Scilit |
|---|---|
| Author | Liang, Xue Su, Yong-Ping Kong, Pei-Yan Zeng, Dong-Feng Chen, Xing-Hua Peng, Xian-Gui Zou, Zhong-Min Xu, Hui |
| Copyright Year | 2010 |
| Description | There is mounting evidence demonstrating that stromal cell derived factor-1 (SDF-1) plays an important role in homing of hematopoietic progenitor cells to bone marrow. This study was aimed to assess whether bone marrow mesenchymal stem cells overexpressing exogenous SDF-1 could synergistically promote the homing of CD34(+) (Cluster of Differentiation [CD]) cells to bone marrow of lethally irradiated severe combined immunodeficiency (SCID) mice. Human SDF-1 complementary Deoxyribonucleic acid (cDNA) was transfected into bone marrow-derived mesenchymal stem cells with recombinant lentiviral vector. The expression of SDF-1 was detected by real-time Polymerase Chain Reaction (PCR) and Enzyme-Linked Immunosorbent Assay (ELISA), and the ex vivo chemotaxis function on CD34(+) cells was measured by coculture system and Transwell system. SDF-1 gene-modified mesenchymal stem cell (MSC) and CD34(+) cells were infused into lethally irradiated SCID mice and the hematopoietic reconstitution in the recipient mice was examined. Messenger ribonucleic acid (mRNA) and protein of SDF-1 in infected MSC were significantly higher than that of the non-infected control MSC (p < 0.05). The infected MSC have significant chemotaxis effect on CD34(+) cells in vitro and promote hematopoietic reconstitution after CD34(+) cell transplantation in vivo. MSC with high-level expression of SDF-1 can synergistically promote hematopoietic reconstitution after CD34(+) cell transplantation in lethally irradiated SCID mice. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/09553000903422555 |
| Ending Page | 237 |
| Page Count | 8 |
| Starting Page | 230 |
| ISSN | 09553002 |
| e-ISSN | 13623095 |
| DOI | 10.3109/09553000903422555 |
| Journal | International Journal of Radiation Biology |
| Issue Number | 3 |
| Volume Number | 86 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2010-03-04 |
| Access Restriction | Open |
| Subject Keyword | Journal: International Journal of Radiation Biology Veterinary Sciences Hematopoietic Function Bone Marrow Lentiviral Vector Cd34 Sdf Vitro Scid Mice Irradiated Scid |
| Content Type | Text |
| Subject | Radiology, Nuclear Medicine and Imaging Radiological and Ultrasound Technology |
