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In-vitro haemocompatibility of dextran-protein submicron particles
| Content Provider | Scilit |
|---|---|
| Author | Kloypan, Chiraphat Suwannasom, Nittiya Chaiwaree, Saranya Prapan, Ausanai Smuda, Kathrin Baisaeng, Nuttakorn Pruß, Axel Georgieva, Radostina Bäumler, Hans |
| Copyright Year | 2019 |
| Description | Blood compatibility is a key requirement to fulfil for intravenous administration of drug and oxygen carrier system. Recently, we published the fabrication of oxidised-dextran (Odex)-crosslinked protein particles by one-pot formulation. In the current study we investigate the haemocompatibility of these Odex - particles including albumin particles (Odex-APs) and haemoglobin particles (Odex-HbMPs). Odex-APs and Odex-HbMPs have a submicron size ranged 800–1000 nm with peanut-like shape and a negative surface charge. In vitro haemocompatibility assays included haemolysis test, indirect phagocytosis test and platelet activation test in human blood. Odex-APs and Odex-HbMPs did not provoke any undesirable effects on the blood cells. Firstly, the ratio of haemolysis after contacted with Odex-crosslinked protein particles were less than 5% and therefore the particles may be considered non-haemolytic. Secondly, the incubation of leukocyte with Odex-APs/HbMPs did not influence the phagocytosis of leukocyte. We conclude that our particles are not recognized by monocytes or granulocytes. Finally, exposure of Odex-APs/HbMPs to platelets did not cause an activation of platelets. Additionally, Odex-HbMP/AP did not enhance or attenuate agonist-induced platelet activation. We conclude that Odex-crosslinked protein particles exhibit a very good haemocompatibility and represent highly promising carriers for drugs or oxygen. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/21691401.2018.1548476?needAccess=true |
| Ending Page | 249 |
| Page Count | 9 |
| Starting Page | 241 |
| ISSN | 21691401 |
| e-ISSN | 2169141X |
| DOI | 10.1080/21691401.2018.1548476 |
| Journal | Artificial cells, nanomedicine, and biotechnology |
| Issue Number | 1 |
| Volume Number | 47 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-01-19 |
| Access Restriction | Open |
| Subject Keyword | Journal: Artificial Cells, Nanomedicine, and Biotechnology Pharmacology and Pharmacy Haemocompatibility Submicron Particles Dextran Hemoglobin |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomedical Engineering Biotechnology Pharmaceutical Science |
