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Improved production of (R)-2-octanol via asymmetric reduction of 2-octanone withOenococcus oeniCECT4730 in a biphasic system
| Content Provider | Scilit |
|---|---|
| Author | Meng, Fantao Xu, Yan |
| Copyright Year | 2010 |
| Description | Oenococcus oeni CECT4730, which catalyses the asymmetric reduction of 2-octanone to (R)-2-octanol with high enantioselectivity, was further studied to exploit its potential for production of (R)-2-octanol in an aqueous/organic solvent biphasic system. Variables such as the volume ratio of aqueous to organic phase $(V_{a}/V_{o}$), buffer pH, reaction temperature, shaking speed, co-substrates and the ratio of biocatalyst to substrate were examined with respect to the molar conversion, the initial reaction rate and the product enantiomeric excess (e.e.). Under the optimized conditions $(V_{a}/V_{o}$=1:1 (v/v), buffer pH=8.0, reaction temperature=30°C, shaking speed=150 rev/min, ratio of glucose to biomass=5.4:l (w/w), ratio of biocatalyst to substrate=0.51:l (g/mol)), the highest space time yield of (R)-2-octanol, 24 mmol $L^{−1}$ per h, and >98% product e.e. were obtained at a substrate concentration close to 1.0 mol $L^{−1}$ after 24 h reduction. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.3109/10242420903536870 |
| Ending Page | 149 |
| Page Count | 6 |
| Starting Page | 144 |
| ISSN | 10242422 |
| e-ISSN | 10292446 |
| DOI | 10.3109/10242420903536870 |
| Journal | Biocatalysis and Biotransformation |
| Issue Number | 2 |
| Volume Number | 28 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2010-01-13 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biocatalysis and Biotransformation Biotechnology and Microbiology Sustainable Science and Technology Oenococcus Oeni (r)-2-octanol Asymmetric Reduction Biphasic System Space Time Yield |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Catalysis Biotechnology |
